Coding
PhzM

Part:BBa_I723024

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)

PhzM

This is the coding region for the first of two Pseudomonas aeruginosa PA01 enzymes required to convert phenazine-1-carboxylic acid (PCA) into pyocyanin.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Janusan Jeyananthan
  • Summary: Added information collated from existing scientific studies

PhzS required for activity in vitro.

Enzyme catalytic activity: phenazine-1-carboxylate + S-adenosyl-L-methionine = 5-methyl-phenazine-1-carboxylate + S-adenosyl-L-homocysteine [1]

PhzM Reaction Diagram.JPG

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 424
    Illegal NgoMIV site found at 428
    Illegal NgoMIV site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ "phzM" n.d Uniprot. Accessed October 3, 2021. https://www.uniprot.org/uniprot/Q9HWH2
[edit]
Categories
//function/biosynthesis/pyocyanin
Parameters
None